Thermo Fisher Scientific TNF alpha Polyclonal Antibody
Thermo Fisher Scientific의 TNF alpha Polyclonal Antibody는 인간 및 마우스 TNF-α를 인식하는 고품질 염소 유래 항체입니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제된 제품입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3–15 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
| Flow Cytometry (Flow) | 2.5 µg per million cells | - |
| ELISA | 0.1–0.4 µg/mL (Detection), 0.2–0.8 µg/mL (Capture) | - |
| Neutralization (Neu) | 0.1–0.4 µg/mL | - |
| Inhibition Assays (IA) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant mouse TNF-alpha (Leu80–Leu235) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609680 |
Product Specific Information
- In sandwich ELISAs, approximately 50% cross-reactivity with recombinant rat TNF-alpha and less than 1% with recombinant human TNF-alpha.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg antibody (LAL method).
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell functions, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimers (two to five noncovalently linked units) but shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies in mice indicate its neuroprotective role and tumor necrosis induction. TNF alpha also contributes to immune responses against bacterial, viral, parasitic, and fungal infections and plays a role in tumor cytolysis or cytostasis, synergistic with interferon-gamma. It is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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