Thermo Fisher Scientific c-Met Monoclonal Antibody (85503)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

5-25 µg/mL

-

Immunocytochemistry (ICC/IF)

5-15 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

85503

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET Glu25-Thr932 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Met,uniProtId:P08581-1,ncbiNodeId:9606,antigenRange:25-932,antigenLength:1390,antigenImageFileName:MA5-23760_c-Met_P08581-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23760_c-Met_P08581-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609610

Product Specific Information

In sandwich ELISAs, no cross-reactivity with recombinant human (rh) EGFR or rhIGF-1 R is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.