Thermo Fisher Scientific IFN gamma Monoclonal Antibody (88928)
쥐 IFN-γ를 인식하는 Mouse IgG1 단클론 항체로, ICC/IF 및 ELISA에 적합합니다. 단백질 A/G 정제 및 동결건조 형태로 제공되며, 보존제 없이 PBS와 트레할로오스를 포함합니다. 교차 반응성이 낮고, 재구성 후 0.5 mg/mL로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 2–8 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 88928 |
| Immunogen | E. coli-derived recombinant rat IFN-γ (Gln23–Cys156) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609804 |
Product Specific Information
- In sandwich ELISAs, cross-reactivity was less than 2% with recombinant mouse IFN-γ, and less than 0.1% with recombinant human and porcine IFN-γ.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits, showing species-specific biological activity.
Human IFN-γ does not cross-react with mouse IFN-γ.
Functions include antiviral and antiproliferative activity, induction of MHC I/II, macrophage activation, and enhancement of immunoglobulin secretion.
IFN-γ acts via IFN-γ receptor I/II and the JAK-STAT pathway, and is regulated by IL2, FGF basic, EGF (upregulated) and vitamin D3 or DMN (downregulated).
Mutations in the IFN-γ gene have been associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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