Thermo Fisher Scientific Cytochrome C Polyclonal Antibody
Western blot, IHC, ICC/IF, Flow Cytometry에 적합한 Rabbit Polyclonal 항체. Human, Mouse, Rat 반응성. 합성 펩타이드(aa 1-50) 면역원 사용. 항원 친화 크로마토그래피 정제. 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Cytochrome C (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7.4) with 40% Glycerol, 0.2% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802823 |
Target Information
Cytochrome C is an electron transport protein located in the mitochondrial intermembrane space, essential for oxidative phosphorylation and ATP production. Upon apoptotic stimuli, cytochrome C is released into the cytosol where it binds apoptotic protease activating factor, leading to activation of procaspase 9 and subsequently caspase 3, a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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