Thermo Fisher Scientific Phospho-STAT5 alpha/beta (Ser726, Ser731) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human STAT5A(Accession P42229), corresponding to amino acid residues around phosphorylated Ser726. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STAT5 alpha/beta,uniProtId:P42229-1,ncbiNodeId:9606,antigenRange:726,antigenLength:794,antigenImageFileName:PA5-104895_STAT5_alphabeta_P42229-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104895_STAT5_alphabeta_P42229-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816368

Product Specific Information

Antibody detects endogenous levels of STAT5 only when phosphorylated at Ser726/Ser731, which site historically referenced as Ser725/Ser730.

Target Information

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of, many cell ligands, such as IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/ BCL-X(L), which suggests the antiapoptotic function of this gene in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.