Thermo Fisher Scientific Phospho-Vinculin (Tyr1133, Tyr1065) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Vinculin only when phosphorylated at Tyr1133 (Isoform 1, P18206-2) or Tyr1065 (Isoform 2, P18206-1).

Target Information

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques. In this assembly, the beta subunit of integrin binds to talin, which binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in linking the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.