Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC-eFluor 780, eBioscience
Mouse CD223 (LAG-3) 단백질을 인식하는 Rat IgG1 단클론 항체로, APC-eFluor 780 형광체가 결합되어 Flow Cytometry 분석에 최적화됨. 활성화된 T 세포 및 NK 세포 표지용으로 적합하며, 광산화에 민감하므로 암조건에서 보관 필요.
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- 47-2231-8x (2개 옵션)
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카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC-eFluor 780, eBioscience
Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), APC-eFluor 780, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioC9B7W (C9B7W) |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637322 |
Additional Formats
- Unconjugated
- APC
- Brilliant Violet 480
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702
- Custom conjugation available
Product Specific Information
The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) expressed by activated alpha/beta-TCR-bearing T cells, subsets of gamma/delta-TCR T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein similar to CD4. Mouse CD223 can be proteolytically cleaved, producing a 54 kDa extracellular fragment and a 16 kDa intracellular fragment. The soluble form may regulate T-cell activation and homeostatic proliferation.
This antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes at ≤1 µg/test (100 µL final volume). Cell count per test should be optimized (10⁵–10⁸ cells/test).
APC-eFluor® 780 emits at 780 nm and is excited with a 633 nm red laser. Ensure instrument compatibility.
Light-sensitive: protect vial and stained samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Performance after fixation should be verified empirically.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
LAG-3 is a 70-kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and negatively regulates T-cell activation and proliferation. Expressed on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells generally express higher levels than CD4+ T cells. Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1 cells) in humans and mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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