Thermo Fisher Scientific CD152 (CTLA-4) Monoclonal Antibody (14D3), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 10 publications

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Product Specifications

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Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 737
• Brilliant UV 805
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

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Product Specific Information

The 14D3 monoclonal antibody reacts with human CD152 (CTLA-4), a protein structurally similar to CD28. It is expressed on activated T cells (and sometimes B cells) and binds CD80 (B7-1) and CD86 (B7-2) with higher affinity than CD28.
CTLA-4 and CD28 deliver opposing signals: CD28 stimulates T cell activation, while CTLA-4 inhibits IL-2 secretion and proliferation. Most CTLA-4 is localized intracellularly due to ER retention motifs.

The 14D3 antibody also recognizes rhesus monkey and exhibits inhibitor activity.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated using the Intracellular Fixation and Permeabilization Buffer Set (Product #88-8824-00) following BestProtocols® Protocol A.
Use 5 µL (0.06 µg) per test in a final volume of 100 µL. Cell count may range from 10⁵–10⁸ cells/test.

Due to intracellular localization, both surface and intracellular CTLA-4 should be assessed for complete detection.

Fluorochrome Properties:

• Excitation: 633–647 nm
• Emission: 780 nm
• Laser: Red Laser
• Light Sensitivity: Protect from light (photo-oxidation sensitive)

Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222), or
• 1-step Fix/Lyse Solution (Product #00-5333)

Filtration: 0.2 µm post-manufacturing filtered.

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Target Information

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor mainly expressed on CD4⁺ T cells.
It binds CD80 and CD86 with higher affinity than CD28, but unlike CD28, CTLA4 inhibits T cell activation and function.
Intracellular CTLA4 is also present in regulatory T cells, contributing to their immunoregulatory role.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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