Thermo Fisher Scientific USP37 Polyclonal Antibody
Rabbit polyclonal antibody against human USP37, validated for WB, IHC(P), and IP. Recognizes residues 930–979 of USP37. Recommended for research use in studying deubiquitination and cell cycle regulation. Supplied as liquid, 0.2 mg/mL, stored at 4°C.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 930 and the C-terminus (residue 979) of Human Ubiquitin Specific Peptidase 37 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Gorilla and Chimpanzee
Target Information
Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of Lys-11-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of Lys-48-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes deubiquitinase activity, preventing degradation of cyclin-A. Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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