Thermo Fisher Scientific USP37 Polyclonal Antibody
USP37 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC(P), IP에 적합. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관. 인간 시료에 반응하며, 고릴라 및 침팬지에서도 예측 반응.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 930 and the C-terminus (residue 979) of Human Ubiquitin Specific Peptidase 37 (USP37, UniProt ID: Q86T82-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Gorilla and Chimpanzee
Target Information
USP37 is a deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during the G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), promoting S phase entry.
It specifically mediates deubiquitination of Lys-11-linked polyubiquitin chains, a ubiquitin-linkage type mediated by the APC/C complex, and also acts on Lys-48-linked polyubiquitin chains in vitro.
Phosphorylation at Ser-628 during the G1/S phase maximizes deubiquitinase activity, preventing cyclin-A degradation.
USP37 plays a crucial role in DNA replication regulation by stabilizing the licensing factor CDT1.
[Source: UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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