Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Brilliant Violet 786, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution ≤ 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 711
• FITC
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. It recognizes both Ly-6E.1 and Ly-6A.2 molecules expressed by hematopoietic stem cells, myeloid populations, and peripheral T/B cells.

Non-activated splenocytes from Ly-6.2 strains (C57BL, SJL, 129, AKR, etc.) show higher D7-positive cell frequency compared to Ly-6.1 strains (BALB/c, C3H, NZB, etc.). Sca-1 expression increases upon activation regardless of haplotype. Sca-1 is a major phenotypic marker for murine hematopoietic progenitor/stem cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells, ≤ 0.5 µg/test (100 µL sample volume)

Brilliant Violet™ 786 (BV786):

• Tandem dye emitting at 786 nm
• Designed for cytometers with 405 nm violet laser
• Protect from light (photo-oxidation sensitive)

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (00-8222-49)
• 1-step Fix/Lyse Solution (00-5333-54)

BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Stem Cell Antigen-1 (Sca-1):

• Member of Ly-6 family
• Marker for mouse hematopoietic stem cells (HSC)
• Expressed in fetal liver, adult bone marrow, peripheral blood, and spleen
• Involved in regulation of B and T cell activation

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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