Thermo Fisher Scientific DWORF Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

1:50-1:250

-

ELISA (ELISA)

1:10,000

-

Immunoprecipitation (IP)

1:50-1:250

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide taken within amino acid region 1-30 on mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5-1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 7.4-7.8, with 30% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity (PubMed:26816378, PubMed:30299255). Does not directly stimulate SERCA pump activity (PubMed:26816378). Enhances sarcoplasmic reticulum Ca(2+) uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN) (PubMed:26816378, PubMed:30299255).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.