Thermo Fisher Scientific PDGF-A/B Monoclonal Antibody (1G10)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

0.01-0.1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

1G10

Immunogen

A recombinant protein of full length human PDGF if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDGF-A/PDGF-B,uniProtId:P01127-1,ncbiNodeId:9606,antigenRange:1-241,antigenLength:241,antigenImageFileName:603-980_PDGF-APDGF-B_P01127-1_House_mouse.svg,antigenImageFileNamePDP:603-980_PDGF-APDGF-B_P01127-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:PDGF-A/PDGF-B,uniProtId:P04085-1,ncbiNodeId:9606,antigenRange:1-211,antigenLength:211,antigenImageFileName:603-980_PDGF-APDGF-B_P04085-1_House_mouse.svg,antigenImageFileNamePDP:603-980_PDGF-APDGF-B_P04085-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Human squamous cell lung carcinoma tissue

Cellular location: Secreted.

Target Information

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of homo/hetero dimeric proteins, PDGF-AA, -BB, -CC, -DD and -AB. The PDGF-AA, -BB and AB bind to alpha type receptor, while beta type receptors bind to PDGF-BB and PDGF-AB. Upon activation by PDGF, the receptors get dimerised and auphosphorylated in several sites on its receptors (Receptor tyrosine kinase) in cytosolic domain to trigger cell signaling through PI3K pathway. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.