Thermo Fisher Scientific EGFP/GFP/Venus Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitute in 100 µL of sterile water.
• Centrifuge to remove any insoluble material.
• Specificity: EGFP/GFP/Venus.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has provided new insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically. The wild-type protein absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications including:

• Monitoring gene expression
• Protein localization studies
• Protein–protein interaction analysis (yeast two-hybrid system)
• Fluorescence energy transfer (FRET) experiments

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.