Thermo Fisher Scientific EGFP/GFP/Venus Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: EGFP/GFP/Venus.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular systems.

As a fusion tag, GFP can be used to localize proteins, study their movement, and analyze the dynamics of subcellular compartments. GFP technology has provided new insights into the physiological activities of living cells.

GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (max 395 nm) and emits green light (peak 508 nm) without the need for additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for a wide range of applications.

Applications include monitoring gene expression, protein localization, studying protein–protein interactions (yeast two-hybrid system), and measuring distances between proteins in FRET experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.