Thermo Fisher Scientific EGFP/GFP/Venus Polyclonal Antibody
EGFP, GFP, Venus 단백질을 인식하는 sheep polyclonal 항체로, Western blot 및 IHC에 사용 가능. 합성 펩타이드 면역원 기반으로 제작되었으며, PBS 용액에 동결건조 형태로 제공. 4°C 단기 보관, 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | - |
| Immunohistochemistry (IHC) | 10–50 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides from EGFP/GFP/Venus protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: EGFP/GFP/Venus.
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in bioluminescence reactions. GFP is widely used as a reporter protein for gene expression in both eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP allows visualization of protein localization, movement, and dynamics of subcellular compartments. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment, absorbing blue light (395 nm) and emitting green light (508 nm). Its fluorescence is stable and species-independent, suitable for diverse applications such as monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid system), and FRET experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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