Thermo Fisher Scientific TOP2A Monoclonal Antibody (OTI1B10), TrueMAB
TOP2A 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1B10). Western blot 및 IHC(P)에서 검증됨. 인간 시료에 반응하며, 동결건조 형태로 제공. DNA 합성과 세포 분열 과정 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1100–1531 of human TOP2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Topo II alpha is involved in DNA synthesis, transcription, and chromosomal segregation during mitosis.
It serves as a specific marker for late S-, G2-, and M-phases in transformed and developmentally regulated normal cells.
Topo II alpha is also associated with tumor cell drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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