Thermo Fisher Scientific DOT1L Monoclonal Antibody (OTI2B10), TrueMAB
DOT1L 단클론 항체로 인간 DOT1L 단백질(aa 1-434)에 특이적 결합. Western blot에 적합하며, 무보존제 동결건조 형태로 제공. 연구용 히스톤 메틸트랜스퍼레이스 분석에 활용 가능.
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Thermo Fisher Scientific DOT1L Monoclonal Antibody (OTI2B10), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–434 of human DOT1L produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
DOT1L is a histone methyltransferase that methylates Lys-79 of histone H3.
It prefers nucleosomes as substrates compared to free histones and binds to DNA.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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