Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody
ELISA 등 다양한 면역분석에 사용 가능한 염소 유래 항-마우스 IgG (H+L) 2차 항체. F(ab')2 프래그먼트 형태로 비특이적 결합 최소화. 친화 크로마토그래피로 정제되어 높은 특이도와 재현성 제공. 비결합형(Unconjugated)으로 다양한 검출 시스템에 유연하게 적용 가능.
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Applications and Tested Dilution
- ELISA (ELISA): 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535983 |
| Target | IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Each lot of antibody is validated for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target molecules. Secondary antibodies enhance versatility by allowing use with multiple detection systems (e.g., HRP, AP, fluorescence) and improve sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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