Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser218, Ser222) Polyclonal Antibody
Phospho-MEK1/MEK2(Ser218,Ser222) 특이적 폴리클로날 항체로 Western blot 및 IHC(P) 검증 완료. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 고순도(>95%) 항체. PBS/glycerol 완충액, 4°C 단기 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2553758 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 43 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003).
In brain tissue, this pathway also plays a key role in synaptic regulation (Adams and Sweatt, 2002).
Activated MEK1 functions as a dual specificity kinase phosphorylating both threonine and tyrosine residues on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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