Thermo Fisher Scientific PSPN Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Persephin. Anti-Human Persephin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Persephin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Persephin (500-P138) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Persephin.

Western Blot:
To detect hPersephin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hPersephin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P138BT-1MG will be provided as 2 × 500 µg.

Target Information

Persephin is a disulfide-linked, homodimeric neurotrophic factor structurally related to GDNF, artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system composed of RET and one of four GFR alpha (α1–α4) receptors. The GFRα4 was first identified in chicken and was later shown to be the preferential binding subunit for persephin. Persephin promotes the survival of ventral midbrain dopaminergic neurons and motor neurons after sciatic nerve axotomy, and, like GDNF, promotes ureteric bud branching. However, in contrast to GDNF and neurturin, persephin does not support the survival of peripheral neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.