Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), Super Bright 600, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 5 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 661 / 737
• Brilliant Violet 421
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 645 / 702 / 780
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 can induce T cell activation, proliferation, or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.125 µg per test (in 100 µL final volume). Cell number per test may range from 10⁵ to 10⁸ cells. Antibody titration is recommended for optimal performance.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Ensure the instrument supports this fluorochrome.

Usage Recommendations:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark, with minimal loss of brightness or FRET efficiency.

Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

T cell receptors (TCRs) enable T cells to recognize peptides presented by MHC class II molecules, essential for adaptive immune responses. Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies against TCR V beta segments have been found in rheumatoid arthritis and lupus patients, potentially modulating inflammatory reactions.
Most human T cells express TCR alpha-beta with CD4 or CD8 (single positive). Some are double negative (DN), and increased DN T cells are observed in autoimmune or immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelium, show distinct antigen recognition and may have antitumor and immunoregulatory roles.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지