Thermo Fisher Scientific B7-H4 Monoclonal Antibody (H74), Super Bright 600, eBioscience
인간 B7-H4 단백질을 인식하는 H74 단클론 항체로, Super Bright 600 형광체가 결합된 제품입니다. 유세포 분석(Flow Cytometry)에 적합하며, 405 nm 레이저로 여기되어 600 nm에서 방출됩니다. 광민감성이 있으므로 암조건에서 보관하며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | - |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H74 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784875 |
Product Specific Information
The H74 monoclonal antibody reacts with human B7-S1 (also known as B7-H4 or B7x). Cross-reactivity with other proteins has not been determined. B7S1 is a member of the B7 family, involved in the costimulatory regulation of T cells, potentially influenced by B cell activation status. BTLA has been suggested as a counter receptor, though further validation is needed.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Pre-diluted and tested by flow cytometry on B7-H4-transfected human cells. Recommended use: 5 µL (0.25 µg) per test (100 µL total volume, 10⁵–10⁸ cells/test).
Super Bright 600 Characteristics:
- Tandem dye excited by 405 nm violet laser; emits at 600 nm (recommended 610/20 bandpass filter).
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright conjugates to minimize non-specific interactions.
- Light-sensitive: protect from light during storage and staining.
- Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57); samples stable up to 3 days at 4°C in dark.
- Excitation: 405 nm / Emission: 600 nm / Laser: Violet.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
B7-H4 (B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity. It delivers inhibitory signals to T-cells, suppressing proliferation, cell cycle progression, and IL-2 production.
- Expressed on activated T-cells, B-cells, monocytes, and dendritic cells.
- Aberrant expression is linked to lung, breast, and ovarian cancers.
- Overexpression may enable tumor immune evasion and progression.
- Potential biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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