Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 600, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 2 publications

Product Specifications

Additional Formats Available

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen.
H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (b, j, k, p, q, s, v) has not been observed.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Recommended use ≤ 0.5 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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