Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 600, eBioscience
마우스 H-2Kd MHC class I 항원을 인식하는 SF1-1.1.1 단클론 항체로, Super Bright 600 형광체 결합 제품입니다. Flow Cytometry 분석에 최적화되어 있으며, 높은 특이성과 안정성을 제공합니다. 405 nm 레이저로 여기되어 600 nm에서 방출됩니다.
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Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Super Bright 600, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SF1-1.1.1 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762780 |
Additional Formats Available
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen.
H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (b, j, k, p, q, s, v) has not been observed.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Recommended use ≤ 0.5 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.
Staining Recommendations:
When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
This protein is an MHC class I alloantigen involved in antigen presentation to T cells expressing CD3/TCR and CD8.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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