Thermo Fisher Scientific Sortilin Polyclonal Antibody
Sortilin 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 Polyclonal 항체. Western blot, IHC, Flow cytometry, Neutralization 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 동결건조 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–10 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 0.25 µg/10^6 cells |
| Neutralization (Neu) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Sortilin (Ser78–Asn755) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605863 |
Product Specific Information
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of antibody (LAL method).
Target Information
Sortilin functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.
It is required for protein transport from the Golgi apparatus to lysosomes and endosomes, independent of the mannose-6-phosphate receptor (M6PR).
Sortilin promotes neuronal apoptosis by mediating endocytosis of proBDNF and proNGFB, and also acts as a receptor for neurotensin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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