Thermo Fisher Scientific WWTR1 Polyclonal Antibody
WWTR1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료에서 반응하며, Hippo 신호전달 경로 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:100 |
| ChIP assay (ChIP) | Assay-dependent |
| in situ PLA (PLA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Human TAZ protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2216633 |
Product Specific Information
Suggested positive controls include 293 whole cell lysate, antigen standard for WWTR1 (transient overexpression lysate), HEK 293 lysate, and human kidney protein lysate.
Target Information
WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator acting as a downstream regulatory target in the Hippo signaling pathway. This pathway plays a key role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
The core cascade involves STK3/MST2 and STK4/MST1 kinases, which, in complex with SAV1, phosphorylate and activate LATS1/2 in association with MOB1. MOB1 subsequently phosphorylates and inactivates YAP1 and WWTR1/TAZ.
WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation, regulates nuclear accumulation of SMADS, and couples them to the transcriptional machinery, including the mediator complex. It is also involved in embryonic stem-cell self-renewal, cell proliferation, and epithelial-mesenchymal transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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