Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI6C12), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases.
It contains a forkhead-associated (FHA) domain that mediates interactions with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 shows cell cycle–dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during mitosis.

Because Ki-67 is absent in G0 cells and increases from G1 through mitosis, it serves as a reliable proliferation marker.
Ki-67 antibodies are widely used to determine the growth fraction in neoplasms, correlating with tumor grade and prognosis.
It is also commonly used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.