Thermo Fisher Scientific BTRC Polyclonal Antibody
BTRC 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 Affinity chromatography 정제, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human BTRC (Accession Q9Y297), corresponding to amino acid residues L37–C87 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2854548 |
Product Specific Information
Antibody detects endogenous levels of total BTRC.
Target Information
This gene encodes a member of the F-box protein family characterized by an approximately 40 amino acid motif, the F-box. F-box proteins are subunits of the ubiquitin protein ligase complex SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
The F-box proteins are divided into three classes:
- Fbws containing WD-40 domains
- Fbls containing leucine-rich repeats
- Fbxs containing other protein-protein interaction modules or no recognizable motifs
The protein encoded by this gene belongs to the Fbws class and contains multiple WD-40 repeats. It is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2, and Drosophila Slimb proteins.
It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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