Thermo Fisher Scientific Glucocorticoid Receptor Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 40 publications 40 publications

Immunohistochemistry (IHC)

-

View 28 publications 28 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 9 publications 9 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 3 publications 3 publications

ChIP assay (ChIP)

-

View 25 publications 25 publications

ChIP-sequencing (ChIP-Seq)

-

View 5 publications 5 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Chicken, Human, Mammal, Mouse, Non-human primate, Rabbit, Rat, Sheep, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Glucocorticoid Receptor (Nr3c1),uniProtId:P04150-1,ncbiNodeId:9606,antigenRange:347-367,antigenLength:777,antigenImageFileName:PA1-511A_Glucocorticoid_Receptor_Nr3c1_P04150-1_Rabbit.svg,antigenImageFileNamePDP:PA1-511A_Glucocorticoid_Receptor_Nr3c1_P04150-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2236340

Product Specific Information

PA1-511A detects glucocorticoid receptor (GR) from human, mouse and rat tissues.

For reconstitution, centrifuge the vial briefly, then add 50 µL PBS to achieve a final antibody concentration of 1 mg/mL.

PA1-511A has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract. Immunocytochemical staining of GR in HeLa cells using PA1-511A results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration.

The PA1-511A immunogen is a synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR. The PA1-511A immunizing peptide (Cat. # PEP-001) is available for use in neutralization and control experiments.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.