Thermo Fisher Scientific mTOR Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Immunogen corresponds to amino acids 1223-1290 of human mTOR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:mTOR,uniProtId:P42345-1,ncbiNodeId:9606,antigenRange:1223-1290,antigenLength:2549,antigenImageFileName:PA1-518_mTOR_P42345-1_Rabbit.svg,antigenImageFileNamePDP:PA1-518_mTOR_P42345-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325371

Product Specific Information

3-8% Tris-Acetate Gel is recommended for Western blot.

PA1-518 detects a predominant band at ~290kD corresponding to mTOR. Other lower MW nonspecific bands of unknown identity were also detected at ~65kD and ~35kD in some cell lysates.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.