Thermo Fisher Scientific KRT8 Monoclonal Antibody (UMAB1), UltraMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ChIP assay (ChIP)

Assay-Dependent

-

Peptide Array (Array)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

UMAB1

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 8,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:91-381,antigenLength:483,antigenImageFileName:UM500001CF_Cytokeratin_8_P05787-1_House_mouse.svg,antigenImageFileNamePDP:UM500001CF_Cytokeratin_8_P05787-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.