Thermo Fisher Scientific KRT19 Monoclonal Antibody (UMAB2), UltraMAB
Human KRT19 단백질을 인식하는 UMAB2 단클론 항체로, Western Blot, IHC, ICC/IF에 적합합니다. 무보존제 동결건조 형태이며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 세포 및 조직 내 Cytokeratin 19 발현 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB2 |
| Immunogen | Recombinant fragment expressed in E. coli corresponding to amino acids 240–390 of human CK19 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product # 89882).
Target Information
Cytokeratin 19 is a member of the intermediate filament protein family, showing biochemical diversity among human epithelial tissues with at least 20 polypeptides. Cytokeratins range from 40–68 kDa and have isoelectric points between 4.9–7.8.
Their expression patterns are characteristic of epithelial type and differentiation stage, aiding in the distinction of epithelial malignancies. Cytokeratin antibodies are valuable in immunohistochemistry, cytopathology, and flow cytometry.
Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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