Thermo Fisher Scientific XPF Monoclonal Antibody (OTI4E11), TrueMAB
XPF 단백질을 표적으로 하는 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합함. 인간 및 생쥐 반응성, 고순도 친화 크로마토그래피 정제. 안정적인 액상 형태로 연구용 DNA 손상 복구 관련 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E11 |
| Immunogen | Full-length human recombinant protein of human ERCC4 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The structure-specific ERCC1/XPF endonuclease complex is involved in the repair of two distinct types of DNA lesions: nucleotide excision repair (NER) for UV-induced lesions and bulky chemical adducts, and recombination repair of interstrand cross-links.
In mammalian cells, XPG cleaves 3′ of the DNA lesion, while the ERCC1/XPF complex makes the 5′ incision. ERCC1/XPF likely plays a role in processing partially repaired recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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