Thermo Fisher Scientific TIM-3 Monoclonal Antibody (1E3)
인간 TIM-3 단백질을 표적으로 하는 Mouse IgG1 단일클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능합니다. Protein A로 정제된 액상 항체로, 장기 보관 시 -20°C에서 보관을 권장합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E3 |
| Immunogen | Recombinant protein corresponding to Human TIM-3 (amino acids 1–89) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% Glycerol, 0.2% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2802487 |
Target Information
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-domain containing-3) is a 281-amino-acid, type I transmembrane glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It contains an IgV-like domain, a mucin-like extracellular domain, and a conserved tyrosine phosphorylation motif in the cytoplasmic region. TIM3 plays roles in macrophage activation, autoimmune disease induction, and regulation of Th1-mediated immune responses, contributing to immune tolerance. It is implicated in experimental autoimmune encephalomyelitis (EAE) and modulates disease severity in mouse models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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