Thermo Fisher Scientific MAP2K4 Monoclonal Antibody (OTI2E4), TrueMAB
Human MAP2K4 단백질을 인식하는 Mouse IgG2b 형 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, PBS와 trehalose buffer에 보존. 연구용으로 MAP2K4 관련 세포 스트레스 및 종양 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E4 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human MAP2K4 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K4 (MEK4) is a serine/threonine kinase belonging to the STE7 family. It phosphorylates and activates JNK1/2 and p38, preferentially targeting tyrosine residues of JNKs.
MAP2K4 mediates various cellular stress and inflammatory responses and plays a key role in carcinogenesis. Loss-of-function mutations have been identified in lung and pancreatic tumors, while pro-oncogenic roles have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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