Thermo Fisher Scientific Phospho-MARCKS (Ser152, Ser156) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MARCKS,uniProtId:P30009-1,ncbiNodeId:10114,antigenRange:152,antigenLength:309,antigenImageFileName:PA1-4629_MARCKS_P30009-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4629_MARCKS_P30009-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2234991

Product Specific Information

This antibody is predicted to react with bovine, chicken, human, mouse, Xenopus and zebrafish based on 100% sequence homology.

This antibody is specific for the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots of rat brain homogenates. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Target Information

MARCKS is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.