Thermo Fisher Scientific KLH Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:10,000–1:20,000

ELISA

• Tested Dilution: 1:10,000–1:20,000

Product Specifications

Product Specific Information

• Positive Control: Full length KLH protein
• Product has been tested in direct ELISA

Target Information

Keyhole limpet hemocyanin (KLH) is a large, heterogeneous glycosylated protein composed of subunits with molecular weights of 350,000 and 390,000, forming aggregates of 4,500,000–13,000,000. Each subunit domain contains two copper atoms that bind a single oxygen molecule (O₂), giving the protein a distinctive blue color when oxygenated.

KLH is highly immunogenic yet safe in humans, making it valuable as a vaccine carrier protein. It cannot be synthesized artificially and is available only as a purified biological product from Megathura crenulata. KLH is widely used as a carrier protein in research, biotechnology, and therapeutic applications.

Haptens—small molecules or peptides—require carrier proteins like KLH to elicit an immune response. KLH’s large size, multiple epitopes, and abundant lysine residues enable efficient hapten coupling and strong immune stimulation. Its evolutionary distance from mammalian proteins minimizes false positives in immunological assays using mammalian models. However, KLH tends to aggregate, which can complicate conjugation and laboratory handling, though aggregates remain immunogenic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.