Thermo Fisher Scientific H3K4me1 Polyclonal Antibody
H3K4me1 변형 히스톤 H3를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도, 핵 내 단백질 검출용. 연구용으로만 사용 가능.
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Thermo Fisher Scientific H3K4me1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Dot Blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within human histone H3 aa 1–49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2913553 |
Product Specific Information
- Positive Control: NCCIT, human liver carcinoma tissue, human colon carcinoma tissue, human pancreas tissue, SHSY5Y
- Subcellular Location: Nucleus
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds DNA to form the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression. The N-terminal tail of histone H3 undergoes these modifications, affecting various cellular processes.
Regulatory Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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