Thermo Fisher Scientific BCL6 Polyclonal Antibody
BCL6 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성 확인. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol 버퍼에 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
| ChIP assay (ChIP) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published species | Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 160–415 of Human BCL6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.27 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2544866 |
Product Specific Information
- Recommended positive controls: Jurkat, K562, THP-1, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12
- Predicted reactivity: Mouse (91%), Rat (91%), Dog (90%), Pig (89%), Rhesus Monkey (97%), Bovine (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas.
Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, and rearrangements have been detected in 33–45% of diffuse large B cell lymphomas.
Detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas, and in nodular, lymphocyte predominant Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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