Thermo Fisher Scientific Sucrose Phosphorylase Polyclonal Antibody, Biotin
Biotin으로 표지된 Sucrose Phosphorylase 폴리클로날 항체로, Western blot, ELISA, IP에 사용 가능. E.coli 유래 항원을 기반으로 제작되었으며, Goat IgG 형식. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sucrose Phosphorylase [E.coli] |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Sucrose Phosphorylase antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the buffer described above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, as well as purified and partially purified Sucrose Phosphorylase [E.coli].
Cross-reactivity against Sucrose Phosphorylase from other sources is unknown.
Target Information
Sucrose phosphorylase catalyzes the reaction:
Sucrose + phosphate → D-fructose + α-D-glucose 1-phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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