Thermo Fisher Scientific Glucoamylase Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,500

-

ELISA (ELISA)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

1:500-1:2,500

-

Product Specifications

Species Reactivity

Fungi

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glucoamylase [Rhizopus]

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown.

Target Information

Rhizopus glucoamylase exists in multiple forms, Gluc 1, Gluc 2, and Gluc 3, all of which hydrolyze gelatinized starch at similar rates, but only the largest one (Gluc 1) is able to adsorb raw starch. Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.