Thermo Fisher Scientific Glucoamylase Polyclonal Antibody, Biotin
Rhizopus 유래 Glucoamylase를 인식하는 Biotin 결합 염소 다클론 항체. Western blot, ELISA, IP에 적합. 고순도 정제 및 안정적 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fungi |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glucoamylase [Rhizopus] |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only before immediate use.
Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis. Immunoelectrophoresis shows a single precipitin arc against anti-Biotin, anti-Goat Serum, and purified or partially purified Glucoamylase [Rhizopus]. Cross-reactivity with Glucoamylase from other sources is unknown.
Target Information
Rhizopus glucoamylase exists in multiple forms (Gluc 1, Gluc 2, Gluc 3), all hydrolyzing gelatinized starch at similar rates. Only Gluc 1 can adsorb raw starch. The enzyme hydrolyzes terminal (1→4)-linked α-D-glucose residues successively from non-reducing ends, releasing β-D-glucose.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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