Thermo Fisher Scientific Glucose Dehydrogenase Polyclonal Antibody, Biotin
Glucose Dehydrogenase를 인식하는 Biotin-conjugated Polyclonal Antibody로, Western blot, ELISA, IP에 사용 가능. Goat 유래 IgG로 제작되었으며 Bacillus 종 효소를 면역원으로 함. 4°C 단기 보관, 장기 보관은 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| ELISA | 1:5,000–1:25,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glucose Dehydrogenase (Bacillus species) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis against the buffer described above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, and purified or partially purified Glucose Dehydrogenase (Bacillus species). Cross-reactivity against Glucose Dehydrogenase from other sources is unknown.
Target Information
Glucose Dehydrogenase catalytic activity:
D-glucose + NAD(P)+ → D-glucono-1,5-lactone + NAD(P)H
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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