Norgen Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
혈장 및 혈청 시료로부터 세포유리 순환 DNA를 빠르고 간편하게 정제하는 스핀 칼럼 키트. 모든 크기의 cfc-DNA 및 바이러스·세균 DNA 분리 가능. PCR, qPCR, NGS 등 다양한 하류 응용과 호환. 억제물 없이 고순도 DNA 확보.
- 브랜드
- Norgen
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Norgen Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
SKU: 55100
For rapid and simple purification of cell-free circulating DNA from plasma/serum samples.
Features and Benefits
- Isolate all sizes of circulating DNA from plasma and serum samples
- Isolate viral and bacterial DNA
- Versatile plasma and serum input volumes
- Concentrate circulating DNA into a flexible elution volume
- Isolate inhibitor-free cell-free circulating DNA
- Compatible with Streck Cell-Free DNA BCT® Tubes
- Purification based on spin column chromatography using Norgen’s proprietary resin matrix
These kits provide a fast, reliable, and convenient spin column method for isolating high-quality, high-purity, inhibitor-free cell-free circulating DNA (cfc-DNA) from plasma/serum samples. The purified cfc-DNA is compatible with PCR, qPCR, methylation-sensitive PCR, Southern blot, microarrays, and NGS.
Background
Plasma/Serum cell-free circulating DNA (cfc-DNA) serves as a potential biomarker for cancers, disease states, and fetal DNA in maternal blood. It is valuable for early diagnosis, prognosis, and therapy monitoring in cancer and autoimmune diseases. Cell-free mitochondrial DNA (cfmtDNA) also shows clinical significance. Typically, cfc-DNA exists as short fragments (<1000 bp).
Cell-free fetal DNA enables non-invasive prenatal testing for fetal sex determination, genetic disorder screening, Rhesus factor, aneuploidy, and preeclampsia identification.
Kit Variants
| Kit Type | Input Volume | Elution Volume | Description |
|---|---|---|---|
| Micro Kit | 10–200 μL | 25–50 μL | For small plasma/serum volumes |
| Mini Kit | 200–500 μL | 25–50 μL | For medium plasma/serum volumes |
| Midi Kit | 1–4 mL | 25–50 μL | Handles large input volumes with two-column process |
| Maxi Kit | 5–10 mL | 25–50 μL | For very large plasma/serum volumes |
Kit Specifications
| Specification | Value |
|---|---|
| Minimum Plasma/Serum Input | 200 μL |
| Maximum Plasma/Serum Input | 500 μL |
| Size of DNA Purified | ≥ 50 bp |
| Elution Volume | 25–50 μL |
| Time to Complete 10 Purifications | 15–20 minutes |
| Average Yields | Variable depending on specimen |
Refer to product manual (page 7) for average yields and DNA quantification methods.
Storage Conditions and Stability
All solutions should be tightly sealed and stored at room temperature.
Kit stability: 2 years from shipment date.
Proteinase K is ready-to-use and stable for up to 2 years in prepared storage buffer.
Kit Components
| Component | Cat. 55500 (Micro, 50 preps) | Cat. 55100 (Mini, 50 preps) | Cat. 55600 (Midi, 20 preps) | Cat. 55800 (Maxi, 10 preps) |
|---|---|---|---|---|
| Binding Buffer B | 40 mL | 2 × 40 mL | 2 × 85 mL + 1 × 12 mL | 2 × 85 mL + 1 × 40 mL |
| Proteinase K | 0.6 mL | 2 × 1 mL | 6.5 mL | 8 mL |
| Solution WN | 18 mL | 18 mL | 18 mL | 18 mL |
| Wash Solution A | 18 mL | 38 mL | 18 mL | 18 mL |
| Elution Buffer B | 8 mL | 8 mL | 30 mL | 30 mL |
| Micro Spin Columns | 50 | 50 | 20 | 10 |
| Mini Spin Columns | - | 50 | - | - |
| Midi Spin Columns | - | - | 20 | - |
| Maxi Spin Columns | - | - | - | 10 |
| Collection Tubes | 50 | 100 | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 100 | 20 | 10 |
| Product Insert | 1 | 1 | 1 | 1 |
Documentation
- Protocol: 55100 Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit (50 Preps)
- Application Note: Assessing the Effect of Simulated Shipping on Cell-Free DNA in Blood Collected in Norgen, Streck and EDTA Tubes
- SDS: 55100 Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
- Flyer: Plasma/Serum Cell-Free Circulating DNA Purification Kits
FAQs
Q: What if a variable speed centrifuge is not available?
A: A fixed speed centrifuge can be used, but reduced yields may occur.
Q: At what temperature should centrifugation be performed?
A: All centrifugation steps at room temperature; 4°C is also acceptable.
Q: Can I perform a second elution?
A: Yes, recommended at 50% volume of first elution and in a separate tube.
Q: Why is DNA yield low?
A: Plasma/Serum samples contain very little cfc-DNA; increase input volume to improve yield.
Q: Is RNase treatment required?
A: No, RNA is not co-purified with DNA using this kit.
Q: Can this kit isolate mtDNA or cfDNA from BALF samples?
A: Yes, both applications are supported.
Citations (Selected)
| Title | Journal | Year |
|---|---|---|
| Plasma Extracellular Vesicle-Derived miR-296-5p is a Maturation-Dependent Rejuvenation Factor | Journal of Extracellular Vesicles | 2025 |
| Exploring mitochondrial DNA copy number in circulating cfDNA | The FASEB Journal | 2024 |
| Monitoring of plasma circulating donor DNA reflects cardiac graft injury | Biomedical Reports | 2024 |
| Plasma cfDNA abundance as a prognostic biomarker in cardiovascular patients | Mechanisms of Ageing and Development | 2024 |
| Multidimensional fragmentomic profiling of cfDNA released from patient-derived organoids | Human Genomics | 2023 |
Related Product
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit
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