
Thermo Fisher Scientific RPL14 Polyclonal Antibody
Human RPL14 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보존. 단기 4°C, 장기 -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human RPL14. Recombinant protein control fragment (Product #RP-102221). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646726 |
Product Specific Information
Immunogen sequence:
KKMRNRIIKN EVKKLQKAAL LKASPKKAPG TKGTAAAAAA AAAAAAAAAK VPAKKITAAS KKAPAQKVPA QKATGQKAAP APKAQKGQKA PAQKAPAPKA S
Highest antigen sequence identity to orthologs:
- Mouse: 76%
- Rat: 73%
Target Information
Ribosomes are organelles that catalyze protein synthesis and consist of a small 40S subunit and a large 60S subunit. These subunits are composed of 4 RNA species and approximately 80 distinct proteins. The RPL14 gene encodes a ribosomal protein that is a component of the 60S subunit and belongs to the L14E family. It contains a basic region-leucine zipper (bZIP)-like domain and is located in the cytoplasm. The gene includes a highly polymorphic trinucleotide (GCT) repeat tract, resulting in variable alanine stretches in the protein. Multiple transcript variants exist, all encoding the same protein. As typical for ribosomal protein genes, processed pseudogenes are dispersed throughout the genome.
References:
Aoki M., Diabetes 45:157–164 (1996)
Tanaka M., Biochem. Biophys. Res. Commun. 243:531–537 (1998)
Yoshihama M., Genome Res. 12:379–390 (2002)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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