Thermo Fisher Scientific PYGL Polyclonal Antibody
Thermo Fisher Scientific의 PYGL Polyclonal Antibody는 인간 PYGL 단백질을 인식하는 토끼 유래 IgG 항체로, WB, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반의 안정적 저장용액을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PYGL (Product #RP-88882) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646168 |
Product Specific Information
Immunogen sequence:
VVAATLQDII RRFKASKFGS TRGAGTVFDA FPDQVAIQLN DTHPALAIPE LMRIFVDIEK LPWSKAWELT QKTFAYTNHT VLPEALERWP VDLVEKLLPR HLEIIYEINQ KHLDRIVALF PKDVDRLRRM SLI
Antigen sequence identity to orthologs:
- Mouse: 92%
- Rat: 93%
Target Information
This gene encodes a homodimeric protein that catalyzes the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. It switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity is regulated by allosteric effectors and hormonal controls.
Humans have three glycogen phosphorylase genes expressed mainly in liver, brain, and muscle. The liver isozyme supports systemic glycemic demands, while the brain and muscle isozymes serve local tissues.
Mutations in the liver glycogen phosphorylase gene cause glycogen storage disease type VI (Hers disease), leading to moderate hypoglycemia, mild ketosis, growth retardation, and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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