Thermo Fisher Scientific PFKP Monoclonal Antibody (OTI3A12), TrueMAB
PFKP 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western Blot, IHC, ICC/IF, Flow Cytometry 등에 적합합니다. 동결건조 형태로 제공되며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A12 |
| Immunogen | Full-length human recombinant PFKP protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein kinases catalyze the transfer of phosphate groups from ATP to substrate proteins, mediating signal transduction in eukaryotic cells. They regulate metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, cell movement, apoptosis, and differentiation.
The protein kinase family includes over 500 members, classified into 8 major groups based on catalytic domain sequences.
The AGC kinase group comprises 63 kinases, including:
- Cyclic nucleotide-regulated kinases (PKA & PKG)
- Diacylglycerol/phospholipid-dependent kinases (PKC)
- RAC/Akt kinases
- ARK family (phosphorylates GPCRs)
- RSK family (phosphorylates ribosomal protein S6)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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