Thermo Fisher Scientific PAX7 Polyclonal Antibody

Thermo Fisher Scientific PAX7 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma, and skeletal muscle satellite cells, and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma, and skeletal muscle satellite cells.
The ~57 kDa band appears enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in this application.
Weak unknown bands of lower molecular weight and at ~110–130 kDa are also detected.
Specificity of the antibody was confirmed in HeLa cells overexpressing full-length PAX7.

Target Information

This gene belongs to the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain.
These genes play critical roles during fetal development and cancer growth.
The specific function of the paired box 7 gene (PAX7) is not fully understood but may involve tumor suppression, as fusion with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma.
Three transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.