Thermo Fisher Scientific PAX7 Polyclonal Antibody
PAX7 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human 및 Mouse 시료 반응성. 고순도 Protein G 정제, 안정적인 PBS/glycerol 저장용액.
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Thermo Fisher Scientific PAX7 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 5 publications |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 3 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | 3 µg | View publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified internal fragment of human recombinant PAX7 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 30% glycerol, 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539886 |
Product Specific Information
Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma, and skeletal muscle satellite cells, and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma, and skeletal muscle satellite cells.
The ~57 kDa band appears enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in this application.
Weak unknown bands of lower molecular weight and at ~110–130 kDa are also detected.
Specificity of the antibody was confirmed in HeLa cells overexpressing full-length PAX7.
Target Information
This gene belongs to the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain.
These genes play critical roles during fetal development and cancer growth.
The specific function of the paired box 7 gene (PAX7) is not fully understood but may involve tumor suppression, as fusion with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma.
Three transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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