Thermo Fisher Scientific ATG16L1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunocytochemistry (ICC/IF)

1:100 - 1:3000

View 1 publication 1 publication

ELISA (ELISA)

1:2,000-1:3,000

-

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminal residues of human ATG16-L1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATG16L1,uniProtId:Q676U5-1,ncbiNodeId:9606,antigenRange:1,antigenLength:607,antigenImageFileName:PA1-18296_ATG16L1_Q676U5-1_Rabbit.svg,antigenImageFileNamePDP:PA1-18296_ATG16L1_Q676U5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070450

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Target Information

Autophagy related protein 16 L is encoded by the ATG16L gene and is located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10). ATG 12 and ATG5 conjugate with each other and undergoes homo oligomerisation with ATG16L to form an 800 kDa complex called the Atg16L complex. This complex facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE) and this ubiquitin-like conjugation system is a widely used marker for autophagy. However, the ATG 16L complex along with other ATGs like ATG 18 and ATG 21 prevent the premature cleavage of ATG8-PE by the cysteine protease, Atg4 until the autophagosome is deemed complete.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.