Thermo Fisher Scientific Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

1:2,000

Immunocytochemistry (ICC/IF)

4 µg/mL

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

IgG gamma 1

Conjugate

Alexa Fluor™ 568 Alexa Fluor™ 568 Alexa Fluor™ 568

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Biotin-XX](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/Biotin-XX/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG1 Cross-Adsorbed/conjugates/PE/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

579/603 nm View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_2535766

Target

IgG1

Cross Adsorption

Against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins and mouse isotypes IgG2a, IgG2b and IgG3 prior to conjugation

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, these goat anti-mouse IgG1 whole secondary antibodies have been affinity purified and cross-adsorbed against mouse IgM, mouse IgA, pooled human sera, purified human paraproteins, and mouse isotypes IgG2a, IgG2b, and IgG3 prior to conjugation. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 568 dye is a bright, orange/red-fluorescent dye with excitation ideally suited to the 568 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.

Product will be shipped at Room Temperature.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.