Thermo Fisher Scientific LC3B Polyclonal Antibody
LC3B 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF, ELISA에 적합합니다. 인간, 마우스, 랫트 반응성. 자가포식 관련 연구 및 세포 내 LC3B 검출에 이상적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–100 of human LC3B (NP_073729.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.95 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804656 |
Product Specific Information
- Positive Samples: C6, NIH/3T3, Mouse brain, Rat brain
- Cellular Location: Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Immunogen Sequence:
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B).
LC3B is associated with microtubule assembly and plays an important role in neurogenesis and autophagy—a process involving degradation of cytoplasmic components.
Three human LC3 isoforms undergo post-translational modifications during autophagy.
Macroautophagy is the major inducible pathway for cytoplasmic turnover and degradation of active enzymes and organelles during nutrient starvation.
LC3B mediates interactions between microtubules and cytoskeletal components and may be involved in cancer, aging, metabolic, neurodegenerative, and cardiovascular/pulmonary disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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